An improved loop-mediated isothermal amplification assay for the detection ofMycoplasma bovis

Journal of Veterinary Medical Science

Masuo SUEYOSHI¹  Ryoko UEMURA¹  Yumiko HIGA¹  Wataru YAMAZAKI¹  Shinya GOTO¹  Yoshitaka GOTO¹ 

[1] Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, 1�?1 Gakuenkibanadai-nishi, Miyazaki 889�?2192, Japan

关键词: cattle;    loop-mediated isothermal amplification (LAMP) assay;    Mycoplasma bovis;    respiratory disease;   

DOI  :  10.1292/jvms.15-0459

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitiveand rapid Mycoplasma bovis detection. A total of 55 bacterial strainswere examined in this study, including 33 M. bovis strains, 14non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains.M. bovis was successfully detected by the LAMP assay within 60 minwithout cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swabsamples were tested directly using our LAMP assays, the previously reported LAMP assay,conventional PCR assay without pre-culture and comparing standard culture methods. Theimproved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively(with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay wasincreased compared to existing methods.

【 授权许可】

Unknown