Detection of circulating tumor cells using GeneScan analysis for antigenreceptor gene rearrangements in canine lymphoma patients

Journal of Veterinary Medical Science

Koichi OHNO²  Hajime TSUJIMOTO²  Hideyuki KANEMOTO²  Kenjiro FUKUSHIMA²  Masashi TAKAHASHI²  Yuko GOTO-KOSHINO²  Saaya HIYOSHI-KANEMOTO²  Kazuyuki UCHIDA¹  Yasuhito FUJINO² 

[1] Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan;Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan

关键词: antigen receptor gene rearrangements;    canine;    circulating tumor cells;    GeneScan;    lymphoma;   

DOI  :  10.1292/jvms.15-0433

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Supplementary materials(1)The presence of circulating tumor cells (CTCs) serves as a prognostic marker andindicator of disease relapse, as well as a means of evaluating treatment efficacy in humanand canine lymphoma patients. As an extension of our previous study for the constructionof clinically useful GeneScan system, we utilized the GeneScan system for detecting CTCsin canine lymphoma patients. Samples from the primary lesion and peripheral bloodmononuclear cells (PBMCs) were obtained from 32 dogs with lymphoma at initial diagnosis.All samples were subjected to polymerase chain reaction (PCR) for antigen receptor generearrangements (PARR) followed by GeneScan analysis. Common clonal rearrangements withidentical amplified fragments were detected in both the primary lesion and PBMCs in 19 ofthe 32 dogs (59.4%). However, the detection rate of CTCs varied among the anatomicalclassification of lymphoma studied. GeneScan analysis following PARR would facilitatestudies on determining the clinical significance of CTCs in canine lymphoma patients.

【 授权许可】

Unknown