Production of GM-CSF Mediated by Cysteine Protease of Der f in Canine Keratinocytes

Journal of Veterinary Medical Science

Naoki CHIMURA²  Harumi KAMISHINA¹  Naho KONDO¹  Tsuyoshi KIMURA²  Sanae SHIBATA¹  Machiko SEKIDO¹  Sadatoshi MAEDA¹  Hiroaki KAMISHINA¹ 

[1] Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan;The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan

关键词: atopic dermatitis;    canine;    Der f;    GM-CSF;    keratinocytes;   

DOI  :  10.1292/jvms.11-0522

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Cited-By(6)House dust mite (HDM) allergens are the most common allergens for induction of IgE-mediated hypersensitivity. Recently, epicutaneous sensitization with HDM allergens has been emphasized in the development of atopic dermatitis (AD) by producing various soluble factors in keratinocytes. Among the soluble factors, GM-CSF is a key molecule that activates Langerhans cells, antigen-presenting cells in the epidermis. In the present study, we investigated the effects of Dermatophagoides farinae (Der f) on GM-CSF production in a canine keratinocyte cell line, CPEK. CPEKs were found to produce GM-CSF upon stimulation by Der f. The GM-CSF production was suppressed by addition of a cysteine protease inhibitor. The present results suggest that cysteine protease-derived Der f may be an initiator of allergic inflammation by inducing the production of GM-CSF in keratinocytes.

【 授权许可】

Unknown