Cyclosporine A Inhibits the mRNA Expressions of IL-2, IL-4 and IFN-γ, but not TNF-α, in Canine Mononuclear Cells

Journal of Veterinary Medical Science

Toshiroh IWASAKI¹  Yasuyuki MOMOI¹  Tetsuro KOBAYASHI¹ 

[1] Department of Veterinary Internal Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology

关键词: atopic dermatitis;    canine;    cyclosporine A;    cytokines;    TNF-α;   

DOI  :  10.1292/jvms.69.887

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(35)Cited-By(24)The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-γ and TNF-α were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and IFN-γ mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-α. These findings are important for assessing the indications of CsA treatment in dogs.

【 授权许可】

Unknown