Leukocyte Emigration in Normal Calves and Calves with Leukocyte Adhesion Deficiency

Journal of Veterinary Medical Science

Hajime NAGAHATA¹  Hiromichi OHTSUKA²  Hiroshi SATO²  Miyuki MASUE²  Takashi KUROSAWA²  Asako MASUYAMA²  Hidetoshi HIGUCHI¹  Megumi YUKI²  Hiroshi NODA¹ 

[1] Department of Animal Health School of Veterinary Medicine, Rakuno Gakuen University;Department of Veterinary Internal Medicine, School of Veterinary Medicine, Rakuno Gakuen University

关键词: BLAD;    bronchoalveolar lumen;    extravasation;    neutrophil;    skin window method;   

DOI  :  10.1292/jvms.59.1143

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(2)The emigration of leukocytes from calves with β2 integrin deficiency (BLAD) into bronchoalveolar spaces and scraped tissues was compared to that of normal calves. Polymorphonuclear neutrophils were found in bronchoalveolar lavage fluid from BLAD-affected calves showing chronic pneumonia. The neutrophils were complement receptor type 3 (CR3)-negative when characterized by flow cytometric analysis using anti-CD18 monoclonal antibody. Chemiluminescent response mediated by CR3 in neutrophils isolated from bronchoalveolar lavage fluid from BLAD-calves showed similar findings obtained from CR3-deficient neutrophils. Neutrophils from normal calves migrated into scraped tissue which was prepared in an upper gluteal surface area, whereas few leukocytes from calves with BLAD migrated to the scraped tissue, evaluated by skin window (Rebuck) method. These findings confirmed the extravasation of CR3-deficient leukocytes into bronchoalveolar lumen in BLAD calves, and demonstrated in vivo characteristics of extravasating property of normal and CR3-deficient neutrophils into scraped tissues.

【 授权许可】

Unknown