Generation of Multinucleated Giant Cells In Vitro from Bovine Monocytes and Macrophages

Journal of Veterinary Medical Science

Reiko NAGATA¹  Kazuhiro YOSHIHARA¹  Yoshihiro MUNETA¹  Shigeki INUMARU¹  Yuichi YOKOMIZO¹  Yasuyuki MORI¹ 

[1] National Institute of Animal Health

关键词: cattle;    GM-CSF;    M-CSF;    MGC;   

DOI  :  10.1292/jvms.66.1065

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(5)The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNγ in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), MGC formations from macrophages were observed only when macrophages were cultured with GM-CSF plus CM. These results indicate that several mechanisms to generate MGC from bovine monocytes-macrophage lineage cells exist, and that GM-CSF is a major mediator of MGC formation in cattle.

【 授权许可】

Unknown