Antibodies to Borna Disease Virus in Infected Adult Rats: An Early Appearance of Anti-p10 Antibody and Recognition of Novel Virus-Specific Proteins in Infected Animal Brain Cells

Journal of Veterinary Medical Science

Kazuyoshi IKUTA¹  Makiko WATANABE¹  Takeshi KOBAYASHI¹  Keizo TOMONAGA¹ 

[1] Department of Virology, Research Institute for Microbial Diseases, Osaka University

关键词: animal brain;    antibody;    Borna disease virus;   

DOI  :  10.1292/jvms.62.775

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(3)The time course for appearance of antibodies to Borna disease virus (BDV) major antigens, p40, p24, p18 and p10 were investigated in BDV-inoculated adult rats by Western blotting. Anti-p10 antibodies were detected in sera as early as anti-p40 and -p24 antibodies at four or five weeks after inoculation. Furthermore, in addition to these major antigens of BDV, the rat serum could detect additional 80-, 58-, 43-, 20-, and 16-kDa proteins in BDV-infected cultured cells and/or animal brain cells by Western blot analysis. Of these proteins, the 20- and 16-kDa proteins were shown to be related to p24 protein by their reactivity with anti-p24 monoclonal antibody. Interestingly, the 58- and 24-kDa were found only in BDV-infected animal brain cells but not in cultured cells. The results in this study could provide a useful information on the mechanism for the viral replication and pathogenesis.

【 授权许可】

Unknown