Effect of different concentrations of melatonin to ram spermatozoa on oxidative stress after cryopreservation

Pesquisa Veterinária Brasileira

Lopes Junior, Edilson S.⁴  Sousa, Pedro H.F.²  Souza, Wildelfrancys L.³  Oliveira, Raimundo P.¹  Toniolli, Ricardo³  Moraes, Elenice A.⁴  Costa, Jonathan M.S.³ 

[1] Bioestatístico Embrapa Semiárido, Petrolina, Brazil;Universidade do Estado da Bahia, Juazeiro, Brazil;Universidade Estadual do Ceará, Fortaleza, Brazil;Universidade Federal do Vale do São Francisco, Petrolina, Brazil

关键词:  Melatonin;    antioxidant;    freezing;    ram;    spermatozoa;    stress;    reactive oxygen species;   

DOI  :  10.1590/S0100-736X2016000700017

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

:The aim was to evaluate the effect of adding different concentrations of melatonin in ram semen diluted after cryopreservation. Ten ejaculates were collected0 from three adult ram (n=30) by means of artificial vagina for sheep. The collected samples were diluted in Tris-egg yolk, to a final concentration 200x106 sptz/mL kept in water bath at 32°C, and melatonin added as treatments: control; 100pM; 100nM; 100μM and 1mM melatonin. Then, the samples were cooled in a cold chamber at 5°C for two hours, in straws of 0.5mL and sealed. They were stored under the liquid nitrogen vapor for 15 minutes to 8cm of liquid blade and frozen with liquid nitrogen. Samples were analyzed for sperm motility, membrane integrity, acrosomal membrane, mitochondrial activity, oxidative stress and quantification of the binding capacity. The variables were subjected to analysis of variance and the means were compared by Tu

【 授权许可】

Unknown