Anti-Tumor Effect of Adenoviral Vector-Mediated p53 Gene Transfer on the Growth of Canine Osteosarcoma Xenografts in Nude Mice

Journal of Veterinary Medical Science

Koichi OHNO¹  Hajime TSUJIMOTO¹  Mitsuhiro YAZAWA¹  Noriko KANAYA²  Yuko GOTO-KOSHINO¹  Nobuo SASAKI²  Ryohei NISHIMURA²  Manabu MOCHIZUKI² 

[1] Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Department of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo

关键词: adenovirus vector;    canine;    mouse model;    osteosarcoma;    P53;   

DOI  :  10.1292/jvms.10-0570

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(36)Cited-By(3)We evaluated the anti-tumor effect of adenoviral vector-mediated p53 gene therapy on the growth of canine osteosarcoma xenografts formed in nude mice. Nude mice were subcutaneously transplanted with cells of 2 P53 mutant canine osteosarcoma cell lines, POS and CHOS. The osteosarcoma xenografts were injected with either an adenoviral vector that expresses canine wild-type P53 (AxCA-cp53) or LacZ (AxCA-LacZ). Tumor growth was significantly inhibited in the xenografts injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS during the observation period of 27 days. An increase of the amount of p21WAF1/CDKN1A mRNA, and the number of apoptotic cells was shown in the tumors injected with AxCA-cp53 in comparison to those injected with AxCA-LacZ or PBS. The present study revealed that the adenoviral vector-mediated p53 gene transfer had an anti-tumor effect in canine osteosarcoma xenografts formed in nude mice.

【 授权许可】

Unknown