Cloning of Canine Osteosarcoma Cells

Journal of Veterinary Medical Science

Kayoko OKUYAMA¹  Kazutoshi NOZAKI¹  Tsuyoshi KADOSAWA¹  Akira TAKEUCHI¹  Hiroyuki NAKAYAMA¹  Nobuo SASAKI¹  Naoaki GOTO¹ 

关键词: canine;    clonal cell;    osteosarcoma;   

DOI  :  10.1292/jvms.57.23

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(7)Six clonal cells were established from a canine osteosarcoma cell line (POS) by a limiting dilution method using feeder cells. Whereas histology of mass developed in nude mice by transplantation of POS cells revealed various cell types, clonal cells were morphologically consistent in size and shape. Doubling time of clonal cells ranged from 30±1.4 to 54±1.3 hr and alkaline phosphatase activity ranged from 0.040±0.001 to 2.61±0.435 μmol/min/mg protein depending on the cell types. When transplanted into nude mice, each clonal cell type formed following four histological types; osteoblastic, chondroblastic, fibroblastic, and undifferentiated types. Since each histological feature was found simultaneously in the primary tumor, osteosarcoma tissue might be a complex of various types of cells having different characteristics. Therefore, POS clonal cells may be useful as a potential tool for the studies of differentiation, Phenotypic expression, and a new therapeutic modality of osteosarcoma.

【 授权许可】

Unknown