CB1 Cannabinoid Receptor Stimulation Modulates Transient Receptor Potential Vanilloid Receptor 1 Activities in Calcium Influx and Substance P Release in Cultured Rat Dorsal Root Ganglion Cells

Journal of Pharmacological Sciences

He-Bin Tang²  Osafumi Yuge¹  Atsuko Inoue²  Masashi Kawamoto¹  Kyoko Oshita¹  Yoshihiro Nakata² 

[1] Department of Anesthesiology and Critical Care, Division of Clinical Medical Science, Graduate School of Biomedical Sciences, Hiroshima University;Department of Pharmacology, Division of Clinical Pharmaceutical Sciences, Programs of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Hiroshima University

关键词: CB1 cannabinoid receptor;    dorsal root ganglion;    protein kinase A;    substance P;    transient receptor potential vanilloid receptor 1;   

DOI  :  10.1254/jphs.FP0040872

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(47)Cited-By(22)Cannabinoids have been reported to have analgesic properties in animals of acute nociception or of inflammatory and neuropathic pain models, but the mechanisms by which they exert such alleviative effects are not yet fully understood. We investigated whether the CB1-cannabinoid-receptor agonist HU210 modulates the capsaicin-induced 45Ca2+ influx and substance P like-immunoreactivity (SPLI) release in cultured rat dorsal root ganglion (DRG) cells. HU210 attenuated the capsaicin-induced 45Ca2+ influx and this effect was reversed by the CB1 antagonist AM251. Treatment of DRG cells with 100 nM bradykinin for 3 h potentiated capsaicin-induced SPLI release accompanied with the induction of cyclooxygenase-2 mRNA expression. The potentiation of SPLI release by bradykinin was reversed by HU210 or the protein kinase A (PKA) inhibitor H-89. HU210 also reduced forskolin-induced cyclic AMP production and forskolin-induced potentiation of SPLI release. These results suggest that CB1 could inhibit either the capsaicin-induced Ca2+ influx or the potentiation of capsaicin-induced SPLI release by a long-term treatment with bradykinin through involvement of a cyclic-AMP-dependent PKA pathway. In conclusion, CB1-receptor stimulation modulates the activities of transient receptor potential vanilloid receptor 1 in cultured rat DRG cells.

【 授权许可】

Unknown