CB1 cannabinoid receptor‐mediated tyrosine phosphorylation of focal adhesion kinase‐related non‐kinase

FEBS Letters

Song, Z.H¹  Zhou, Dan¹ 

[1] Department of Pharmacology and Toxicology, School of Medicine, University of Louisville, Louisville, KY 40292, USA

关键词: CB1 cannabinoid receptor;    Mouse neuroblastoma N1E-115 cell;    HU-210;    Focal adhesion kinase-related non-kinase;    Focal adhesion kinase;    Phosphorylation;    FAK;    focal adhesion kinase;    FRNK;    focal adhesion kinase-related non-kinase;    ODN;    oligodeoxynucleotide;    DMEM;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/S0014-5793(02)03091-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of cannabinoid on the tyrosine phosphorylation of focal adhesion kinase (FAK) and focal adhesion kinase-related non-kinase (FRNK) was investigated in differentiated mouse neuroblastoma N1E-115 cells. HU-210, a potent cannabinoid agonist, elicited a time-dependent enhancement of tyrosine phosphorylation of FRNK, but not FAK. Pretreatment of cells with antisense oligodeoxynucleotide targeting CB1 cannabinoid receptor abolished HU-210-induced FRNK tyrosine phosphorylation. In addition, pretreatment of cells with 8-Br-cAMP also blocked HU-210-induced FRNK tyrosine phosphorylation. These data demonstrated that HU-210 induces FRNK tyrosine phosphorylation by activating G_i-coupled CB1 cannabinoid receptor in N1E-115 cells. This newly discovered, cannabinoid-induced FRNK tyrosine phosphorylation might be a novel mechanism for cannabinoid-induced functional changes.

【 授权许可】

Unknown