ORF13 in the Type III secretion system gene cluster of Edwardsiella tarda binds to the mammalian factor Cugbp2

Diseases of Aquatic Organisms

Toshihiro Nakai¹  Yusuke Takeuchi¹  Jun Okuda¹  Masashi Yasuda¹ 

关键词: Edwardsiella tarda;    Type III secretion system;    ORF13;    Mammalian factor Cugbp2;   

DOI  :  10.3354/dao02987

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The Type III secretion system (TTSS) is essential for the intracellular replication of Edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the TTSS gene cluster is required for intracellular replication and virulence of E. tarda. Here, we show that under TTSS-inducing conditions, the protein ORF13 was secreted into culture supernatant. Then, using a yeast 2-hybrid screen, we show that the mammalian factor Cugbp2, which regulates apoptosis in breast cancer cells, directly interacts with ORF13. A pull-down assay revealed that ORF13 binds to the C-terminal region of Cugbp2. Our results suggest that ORF13 may facilitate E. tarda replication in phagocytes by binding to Cugbp2.

【 授权许可】

Unknown