期刊论文详细信息
Endocrine Journal
New Analytical Method for Pancreas and Liver Regeneration: Normalization of Streptozotocin-Induced Hyperglycemia by Retrograde Injection of Insulin Producing Cells
SOJI KASAYAMA1  SATORU SUMITANI1  XIN XU1  HARUHIKO KOUHARA1  HIROSHI SAITO1  TETSUHIRO KITAMURA1  ICHIRO KAWASE1  NOBUYUKI ASANUMA1 
[1] Department of Molecular Medicine, Osaka University Medical School
关键词: TCPHI;    Cholecystic duct;    Pancreas;    Liver;   
DOI  :  10.1507/endocrj.49.449
学科分类:内分泌与代谢学
来源: Japan Endocrine Society
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【 摘 要 】

References(32)We established a new analytical system in which functioning cells were transplanted directly into the pancreas and liver. The retrograde transplantation of beta cell line, Min6 cells, into the streptozotocin-diabetic mice normalized plasma glucose and insulin levels. The injected cells were protected from pancreatic enzymes with enzyme inhibitor. Blood glucose decreased gradually over 10 days and the diabetic mice recovered weight at the same time. Intraperitoneal glucose tolerance test showed that the peak of plasma glucose of the transplanted mice was less than half that of the control. The insulin secretion of the transplanted mice was recovered and stimulated 4.6 times from the basal secretion. Histological analyses showed that the pancreas and liver were characterized by Min6 cell clusters dispersed throughout the organs. Min6 cells were detected near the pancreatic or bile ducts. It is suggested that the injected cells obstructed the peripheral ducts where they settled. The weight of pancreas and liver did not differ significantly in either Min6 transplanted or the control mice. The metabolic effects on the weights of these organs appeared the same in both groups. This is the first report that cells transplanted via ducts into the pancreas and liver performed their biological function. Our transplantation model makes possible the in vivo analysis of the regeneration machinery of the pancreas and liver.

【 授权许可】

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