Detection of Marek's Disease Virus Serotype 1 (MDV1) Glycoprotein D in MDV1-Infected Chick Embryo Fibroblasts

Journal of Veterinary Medical Science

Takeshi MIKAMI¹  Yukinobu TOHYA¹  Ken MAEDA¹  Yasushi KAWAGUCHI¹  Mitsuru ONO¹  Hyung-Kwan JANG¹  Masahiro NIIKURA¹ 

关键词: glycoprotein D;    Marek's disease virus serotype 1;   

DOI  :  10.1292/jvms.58.777

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(5)Chick embryo fibroblasts (CEFs) infected with three strains of Marek's disease virus serotype 1 (MDV1), GA, Md5 and JM, were subjected to indirect immunofluorescence assay with monoclonal antibodies (MAbs) against MDV1 homolog of glycoprotein D (MDV1 gD) of herpes simplex virus. By the MAbs, a number of MDV1 gD-positive cells were detected in CEFs infected with GA, whereas only a few and no positive cells were detected in CEFs infected with Md5 and JM, respectively. The MDV1 gD in GA-infected CEFs was recognized as the band of 64 kDa in immunoblot analysis using one of the MAbs. This is the first report that the MDV1 gD was detected in MDV1-infected cell cultures.

【 授权许可】

Unknown