Characterization of Canine Herpesvirus Glycoprotein D (Hemagglutinin)

Journal of Veterinary Medical Science

Kentaro FUJITA³  Takeshi MIKAMI³  Yukinobu TOHYA²  Ken MAEDA¹  Yasushi KAWAGUCHI³  Mitsuru ONO³  Naoaki YOKOYAMA³  Xuenan XUAN⁴ 

[1] Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University;Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University;Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo;Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine

关键词: canine herpesvirus;    feline herpesvirus type 1;    glycoprotein D;    hemagglutinin;   

DOI  :  10.1292/jvms.59.1003

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(38)Cited-By(4)Glycoprotein D (gD) of canine herpesvirus (CHV) YP2 strain was expressed in COS-7 and insect (Spodoptera frugiperda; Sf9) cells. The gDs expressed in COS-7 and Sf9 cells reacted with a panel of monoclonal antibodies (MAbs) against CHV gD (hemagglutinin) and an MAb 25C9 against feline herpesvirus type 1 (FHV-1) gD by indirect immunofluorescence assay, and possessed a molecular weight (MW) of approximately 51-55 and 41-46 kilodalton (kDa), respectively, when examined by immunoblot analysis. After treatment with tunicamycin, the MW of the gD expressed in Sf9 cells became approximately 37 kDa. By hemadsorption (HAD) tests using canine or feline red blood cells (RBC), COS-7 cells expressing CHV gD adsorbed only canine RBC, but not feline RBC, whereas control COS-7 cells expressing FHV-1 gD adsorbed feline RBC, but not canine RBC. By hemagglutination (HA) tests, lysates of Sf9 cells expressing CHV gD agglutinated canine RBC, but not feline RBC. These HA and HAD activities were inhibited by HA-inhibition MAbs against CHV gD. Control lysates of Sf9 cells expressing FHV-1 gD agglutinated only feline RBC. Serum from mice inoculated with lysates of Sf9 cells expressing CHV gD possessed a high titer of virus-neutralizing activities against CHV infection. These results indicated that CHV gD is structurally similar to FHV-1 gD, but is functionally different from FHV-1 gD.

【 授权许可】

Unknown