Characterization of the Promoter of the Mouse Prohormone Convertase PC2 Gene

Endocrine Journal

KISHIO NANJO¹  TADASHI HANABUSA¹  MACHI FURUTA¹  SHINYA OHAGI¹  HITOMI TATSUTA¹  HIROTO FURUTA¹  TOKIO SANKE¹ 

[1] The First Department of Medicine, Wakayama University of Medical Science

关键词: PC2;    Mouse;    Promoter;    CAT assay;   

DOI  :  10.1507/endocrj.45.569

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(15)Cited-By(1)Prohormone convertase 2 (PC2) is a member of a family of mammalian subtilisin-like endoproteases that are involved in the processing of prohormones, neuropeptides and many other precursor derived proteins. The expression of PC2 is restricted to neuroendocrine tissues such as pancreatic islets, the pituitary and the brain. To understand the regulation of the PC2 gene, we cloned and characterized the promoter region of the mouse PC2 gene. The transcriptional start site of the mouse PC2 gene is identical to that of the human. There is 79% identity in the sequences of the promoter regions between the mouse and human PC2 genes. The mouse PC2 gene, like the human, does not have a TATA-like motif in the region just upstream of the start of the transcription. Studies with promoter- reporter gene, chrolamphenicol acetyltransferase (CAT), constructs showed that the region from -400 to -170bp was necessary for high level expression of the mouse PC2 gene in the βTC-3 insulinoma cells.

【 授权许可】

Unknown