期刊论文详细信息
Endocrine Journal
Characterization of the Promoter of the Mouse Prohormone Convertase PC2 Gene
KISHIO NANJO1  TADASHI HANABUSA1  MACHI FURUTA1  SHINYA OHAGI1  HITOMI TATSUTA1  HIROTO FURUTA1  TOKIO SANKE1 
[1] The First Department of Medicine, Wakayama University of Medical Science
关键词: PC2;    Mouse;    Promoter;    CAT assay;   
DOI  :  10.1507/endocrj.45.569
学科分类:内分泌与代谢学
来源: Japan Endocrine Society
PDF
【 摘 要 】

References(15)Cited-By(1)Prohormone convertase 2 (PC2) is a member of a family of mammalian subtilisin-like endoproteases that are involved in the processing of prohormones, neuropeptides and many other precursor derived proteins. The expression of PC2 is restricted to neuroendocrine tissues such as pancreatic islets, the pituitary and the brain. To understand the regulation of the PC2 gene, we cloned and characterized the promoter region of the mouse PC2 gene. The transcriptional start site of the mouse PC2 gene is identical to that of the human. There is 79% identity in the sequences of the promoter regions between the mouse and human PC2 genes. The mouse PC2 gene, like the human, does not have a TATA-like motif in the region just upstream of the start of the transcription. Studies with promoter- reporter gene, chrolamphenicol acetyltransferase (CAT), constructs showed that the region from -400 to -170bp was necessary for high level expression of the mouse PC2 gene in the βTC-3 insulinoma cells.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201911300832013ZK.pdf 932KB PDF download
  文献评价指标  
  下载次数:1次 浏览次数:19次