Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and it's application in an epidemiological survey in Brazil

Pesquisa Veterinária Brasileira

Araújo, Fábio S.³  Araújo, Flábio R.³  Madruga, Cláudio R.¹  Queiróz, Rosângela A.¹  Miguita, M.¹  Umaki, Adriana C.S.⁴  Carvalho, Cristiano M.E.¹  Crocci, Adalberto J.²  Marques, Ana Paula C.¹ 

[1] Embrapa Gado de Corte, Campo Grande, Brasil;Universidade Estadual Paulista, Botucatu, Brasil;Universidade para o Desenvolvimento do Estado e da Região do Pantanal, Campo Grande, Brasil;Universidade Católica Dom Bosco, Campo Grande, Brasil

关键词: Babesia bigemina;    ELISA;    serological tests;    cattle;    Pantanal.;   

DOI  :  10.1590/S0100-736X2001000200005

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

.- Madruga C.R., Marques A.P.C., Araújo F.R., Miguita M., Carvalho C.M.E., Araújo F.S., Umaki A.C.S., Crocci A.J. & Queiróz R.A. 2001. Evaluation of an ELISA for detection of antibodies to Babesia bigemina in cattle and it's application in an epidemiological survey in Brazil. Pesquisa Veterinária Brasileira 21(2):72-76. Embrapa Gado de Corte, Rodovia BR 262 Km 4, Cx. Postal 154, Campo Grande, MS 79002-970, Brazil.An indirect enzyme-linked immunosorbent assay (ELISA) using a crude antigen was evaluated for its performance to detect Babesia bigemina antibodies. The sensitivity and specificity were 98.0% and 99.0%, respectively. In agreement with the high specificity, no cross-reactions were verified with sera from calves inoculated three times with 107 Babesia bovis organisms. With regard to the comparison of ELISA and indirect fluorescent antibody test (IFAT) in detecting antibodies against B. bigemina in calves experimentally infected with five Brazilian geographical isolates of this hemoparasite, IFAT was able to detect antibodies one day earlier in most of the calves' sera. There was a good agreement between results shown by ELISA and IFAT with sera from an enzootically stable area (k=0.61). However, there was no agreement between these serological tests with sera from an enzootically unstable area (k=0.33). The ELISA was employed in an epidemiological survey using with 1,367 sera from four counties in the Pantanal of Mato Grosso do Sul and characterized this region as an enzootically stable area, since the prevalences ranged from 87.7 to 98.9%. Therefore, this ELISA with high sensitivity, specificity and performance similar to IFAT can be employed in serological diagnosis of B. bigemina.INDEX

【 授权许可】

CC BY-NC