Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale

Pesquisa Veterinária Brasileira

Cláudio R. Madruga²  Ana Paula C. Marques²  Cássia Rejane B. Leal²  Cristiano M.e. Carvalho²  Flábio R. Araújo¹  Raul H. Kessler² 

[1] ,Empresa Brasileira de Pesquisa Agropecuária Gado de Corte Campo Grande MS ,Brasil

关键词: Anaplasma marginale;    ELISA;    serological tests;    cattle;    Anaplasma marginale;    ELISA;    testes sorológicos;    bovino;   

DOI  :  10.1590/S0100-736X2000000300004

来源: SciELO

【 摘 要 】

A rapid indirect enzyme-linked immunosorbent assay (ELISA) was developed for measuring antibodies against Anaplasma marginale using a partially soluble antigen prepared from semi-purified initial bodies from erythrocytes with 80.0% of rickettsiaemia. This technique utilized alkaline phosphatase and p-nitrophenyl phosphate as reaction indicators. The high sensitivity (100.0%) was confirmed with sera from 100 calves experimentally-infected with A. marginale. All of these animals showed seroconversion before or at the same time of the first rickettsiaemia or even when it was not detected. Also the elevated specificity (94.0%) was confirmed by the low percentage of cross-reactions with sera from animals experimentally-infected with Babesia bigemina and Babesia bovis (1.4 and 6.6%, respectively). Performances of ELISA and indirect fluorescent antibody test (IFAT) with 324 sera from enzootically stable area did not show statistical difference (P>0.05), since the former showed 96.9% and the latter 97.2% of positive reactions. The advantage of this ELISA is a shorter execution time than others developed until now, allowing more samples to be analyzed.

【 授权许可】

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