B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish

Diseases of Aquatic Organisms

Anne Marie Szilvay¹  Audun H. Nerland¹  Håvard Henriksen¹  Kjersti B. Mézeth¹  Sonal Patel¹ 

关键词: Fish nodavirus;    Atlantic halibut;    Atlantic cod;    B2 protein;    Viral nervous necrosis;   

DOI  :  10.3354/dao02015

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Betanodavirus infects both larvae and juvenile fish and can cause the disease viral encephalopathy and retinopathy (VER). During an acute outbreak of VER, infected individuals display several clinical signs of infection, i.e. abnormal swimming pattern and loss of appetite. Betanodaviruses can also cause chronic or persistent infection where the infected individuals show no clinical signs of infection. During infection the viral sub-genomic RNA3 and the RNA3-encoded B2 protein are expressed. Antibodies against the B2 protein from Atlantic halibut nodavirus were raised and used together with antibodies against the capsid protein to detect the presence of these 2 viral proteins in infected cells in culture and at different stages of infection in Atlantic halibut Hippoglossus hippoglossus and Atlantic cod Gadus morhua. The B2 protein was detected in recently infected, but not in chronically infected fish. Results suggest that the detection of B2 may be used to discriminate a recent and presumably active infection from a chronic and presumably persistent infection.

【 授权许可】

Unknown