Pathogenicity of nodavirus strains from striped jack Pseudocaranx dentex and Atlantic halibut Hippoglossus hippoglossus, studied by waterborne challenge of yolk-sac larvae of both teleost species

Diseases of Aquatic Organisms

Toshihiro Nakai¹  Sindre Grotmol¹  Geir K. Totland¹  Yuseku Morita¹  Toyohiro Nishioka¹ 

关键词: Striped jack;    Atlantic halibut;    Fish nodavirus;    Pathogenicity;    Virulence;    Host specificity;    Immunohistochemistry;   

DOI  :  10.3354/dao038169

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The present study shows that differences in pathogenicity exist among fish nodavirus strains. In challenge trials, a Japanese strain (SJ93Nag) was highly virulent to larvae of the striped jack Pseudocaranx dentex but replicationwas not detected in larvae of Atlantic halibut Hippoglossus hippoglossus at 6°C. Conversely, a Norwegian nodavirus strain (AH95NorA) that was highly virulent to the Atlantic halibut larvae did not replicate in striped jack larvae at 20°C.Occurrence of the disease viral encephalopathy and retinopathy (VER) and cumulative mortality were significantly different in the 2 species when challenged with the 2 nodavirus strains. The presence of nodavirus in nervous tissue was monitored byimmunohistochemical methods. Our results support the view that the genetic diversity among nodavirus strains reflects the existence of different viral phenotypes which may be adapted to infect different host species and/or for replicating at differenttemperatures. Fish nodaviruses represent surveyable pathogens well suited for studying the relation between viral genotypic and phenotypic properties such as host specificity, temperature optima, neuroinvasiveness and neurovirulence.

【 授权许可】

Unknown