Flow Cytometric Analysis and Immunohistochemistry of Delayed-Type Hypersensitivity Responses in Mice Immunized with Rat Hepatocytes

Journal of Veterinary Medical Science

Makoto YOKOSUKA¹  Michinori TANAKA¹  Shuichi FURUSAWA¹  Sanenori NAKAMA¹  Kazumasa KONDO¹  Koichi MAMBA¹  Shigeyuki TANABE¹  Yasuho TAURA¹  Yoshikazu HIROTA¹  Munekazu NAKAICHI¹ 

关键词: DTH response;    flow cytometric analysis;    immunohistochemistry;    xenohepatocyte;   

DOI  :  10.1292/jvms.57.245

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Delayed-type hypersensitivity (DTH) responses to rat hepatocytes (HCs) in mice were investigated by flow cytometric analysis and immunohistochemistry with monoclonal antibodies directed against murine class II, CD4, and CD8 antigens. Mice were immunized subcutaneously (s.c.) with 106 rat HCs (referred to as s.c.-immunized mice), and control mice were injected s.c. with sterile Hanks' solution (non-immunized mice). Four days later, 105 rat HCs were injected into the footpad of s.c.-immunized mice and non-immunized mice. The DTH response in s.c.-immunized mice significantly increased after challenge when compared to that in non-immunized mice. The numbers of class II+, CD4+, and CD8+ cells in the footpad, and CD4+ and CD8+ cells in the inguinal lymph node of s.c. -immunized mice significantly increased during the DTH response. An increase in the number of CD4+ cells in the footpad of s.c.-immunized mice after challenge was more significant than that of non-immunized mice. The number of CD4+ cells increased more markedly in the footpad of s.c.-immunized mice as compared to that of CD8+ cells. Furthermore, immunohistochemical studies of the footpad of s.c.-immunized mice showed more severe infiltration of CD4+ cells rather than CD8+ cells at the injection site of rat HCs. These results suggest that the DTH response in the footpad of mice immunized with rat HCs is associated with severe infiltration of CD4+ cells.

【 授权许可】

Unknown