Nucleobindin 2 (NUCB2) in human endometrial carcinoma: a potent prognostic factor associated with cell proliferation and migration

Endocrine Journal

Kiyoshi Ito²  Kiyoshi Takagi¹  Mika Watanabe⁴  Sota Tanaka³  Hironobu Sasano⁵  Takashi Suzuki¹  Yasuhiro Miki²  Chiaki Hashimoto³ 

[1] Departments of Pathology and Histotechnology, Tohoku University Graduate School of Medicine, Sendai, Japan;Department of Disaster Obstetrics and Gynecology, International Research Institute of Disaster Science, Tohoku University, Sendai, Japan;Departments of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan;Department of Pathology, Tohoku University Hospital, Sendai, Japan;Departments of Anatomic Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan

关键词: NUCB2;    Endometrial cancer;    Prognosis;    Biomarker;    Immunohistochemistry;   

DOI  :  10.1507/endocrj.EJ15-0490

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(38)Nucleobindin 2 (NUCB2) is a multifunctional protein containing several functional domains, and associated with wide variety of biological process such as food intake and energy homeostasis.Recently, NUCB2 has been implicated in not only normal human tissues but also some kinds of human malignancies.However, its clinical and/or biological significance has largely remained unknown in endometrial carcinomas.We therefore immunolocalized NUCB2 protein in 87 endometrial carcinoma tissues and examined its clinical significance.NUCB2 immunoreactivity was detected in 19 out of 87 (22%) of endometrial carcinoma cases examined, and positively correlated with Ki67 labeling index, while there was no significant correlation between NUCB2 and stage, histological grade, and progesterone receptor status.Furthermore, NUCB2 immunoreactivity was significantly correlated with increased risk of recurrence and worse clinical outcome regardless of stage or histological grade.Subsequent multivariate analyses did reveal that NUCB2 immunoreactivity was an independent prognostic factor for both disease-free survival and endometrial cancer specific survival.In vitro experiments demonstrated that knockdown of NUCB2 using specific siRNA for NUCB2 significantly impaired cell proliferation and migration of the endometrial carcinoma cell lines, Ishikawa and Sawano cells, and that nesfatin-1 treatment significantly promoted cell proliferation and migration in Ishikawa cells.These findings possibly suggested that NUCB2 and/or nesfatin-1 had pivotal roles in the progression of endometrial carcinomas.Immunohistochemical NUCB2 status may therefore serve as a potent biomarker for endometrial carcinomas.

【 授权许可】

Unknown