Synip phosphorylation is required for insulin-stimulated Glut4 translocation and glucose uptake in podocyte [Rapid Communication]

Endocrine Journal

Tetsurou Satoh⁴  Eijiro Yamada⁴  Hiroki Takahashi⁴  Tsugumichi Saito⁴  Junichi Okada¹  Masatomo Mori⁵  Shuichi Okada⁴  Koshi Hashimoto²  Kihachi Ohshima³  Masanobu Yamada⁴ 

[1] Tottori University Faculty of Medicine, Tottori 683-8503, Japan;Department of Metabolic Preemptive Medicine, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8510, Japan;Gunma University Health and Medical Center, Gunma 371-8511, Japan;Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan;Kitakanto obesity and metabolic research institute, Gunma 379-2311, Japan

关键词: Synip;    Podocyte;    Insulin;    Glucose transporter 4;    Syntaxin4;   

DOI  :  10.1507/endocrj.EJ14-0099

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(15)Cited-By(1)Previously we reported that the phosphorylation of Synip on serine 99 is required for Synip dissociation from Syntaxin4 and insulin-stimulated Glut4 translocation in cultured 3T3-L1 adipocytes. We also reported that the dissociated Synip remains anchored to the plasma membrane by binding to Phosphatidylinositol (3,4,5)-triphosphate. Recently Synip was reported to arrest SNARE-dependent membrane fusion as a selective t-SNARE binding inhibitor. In this study, we have found that Synip is expressed in podocytes although at a somewhat lower level than in adipocytes. To determine whether phosphorylation of Synip on serine 99 is required for insulin-stimulated Glut4 translocation and glucose uptake in podocytes we expressed a phosphorylation deficient Synip mutant (S99A-Synip) that inhibited insulin-stimulated Glut4 translocation and 2-deoxyglucose uptake in adipocytes. We conclude that serine 99 phosphorylation of Synip is required for Glut4 translocation and glucose uptake in both adipocytes and podocytes, suggesting that defects in Synip phosphorylation may underlie insulin resistance and associated diabetic nephropathy.

【 授权许可】

Unknown