Two Types of Cation Channel Activated by Stimulation of Muscarinic Receptors in Guinea-Pig Urinary Bladder Smooth Muscle

Journal of Pharmacological Sciences

Hayato Matsuyama¹  Masanobu Kohda²  Masakazu Nishimura³  Noriyuki Masuda²  Masayuki Yamamoto³  Toshihiro Unno³  Seiichi Komori³  Toshiaki Ishii³ 

[1] Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University, Japan;Pharmacology Research Labs., Drug Discovery Research, Astellas Pharma Inc., Japan;Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Japan

关键词: cation channel;    muscarinic receptor;    carbachol;    bladder smooth muscle;    guinea pig;   

DOI  :  10.1254/jphs.08138FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(38)Cited-By(3)The present study, aiming to elucidate ion channel mechanisms underlying muscarinic receptor–induced depolarization, has characterized membrane currents induced by carbachol in single guinea-pig urinary bladder myocytes. Application of carbachol to cells that were voltage-clamped at −50 mV produced an atropine-sensitive, biphasic inward current consisting of an initial peak followed by a smaller sustained phase. Replacing the extracellular Na+ and intracellular Cl− with impermeable tris+ and glutamate−, respectively, demonstrated that the biphasic current is entirely composed of cation currents. Its initial peak phase was abolished by buffering intracellular Ca2+ to a constant level of 100 nM or depleting intracellular Ca2+ stores, and it was mimicked by the Ca2+ releaser caffeine. Ca2+ entry evoked by voltage steps in the sustained phase induced no noticeable change, indicating that this phase of cation current is insensitive to a rise of [Ca2+]i. These results demonstrate that muscarinic receptor stimulation invokes the openings of two types of cation channel, a Ca2+-activated and a receptor-operated type; the former channels are gated by a rise in [Ca2+]i upon intracellular Ca2+ release, and the latter are gated through other muscarinic receptor–coupled signal transduction mechanisms.

【 授权许可】

Unknown