A Novel Inhibitor of Hypertonicity-Induced Cation Channels in HeLa Cells

The Journal of Physiological Sciences

Yasunobu Okada¹  Frank Wehner²  Tomohiro Numata¹ 

[1] Department of Cell Physiology, National Institute for Physiological Sciences;Department of Systemic Cell Biology, Max-Planck-Institute of Molecular Physiology

关键词: HeLa cells;    cell volume regulation;    cation channel;    flufenamic acid;    2-aminoethoxydiphenyl borate;   

DOI  :  10.2170/physiolsci.SC003007

学科分类：生理学

来源: Springer

【 摘 要 】

References(16)Cited-By(4)Despite the paramount significance of hypertonicity-induced cation channels (HICCs) in cell proliferation and apoptosis, a detailed analysis of these processes is hindered by the very limited number of HICC blockers available. Here, 2-aminoethoxydiphenyl borate (2-APB) is introduced as a novel and effective inhibitor of the HICC in HeLa cells. Its efficiency is defined with reference to flufenamic acid (as the most potent blocker so far), both, in whole-cell patch-clamp recordings and in measurements of cell volume regulation.

【 授权许可】

Unknown