Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles

Bioengineering

Gracielle Ferreira Andrade¹  Wellington Marcos da Silva¹  Giovanna Gomes Lara¹  Marcelo Fernandes Cipreste¹  Edésia Martins Barros de Sousa¹ 

[1] Centro de Desenvolvimento da Tecnologia Nuclear—CDTN—Avenida Presidente Antônio Carlos, 6.627-Campus UFMG, Belo Horizonte CEP 31270-901, Minas Gerais, Brazil;

关键词: MCM-41;    flufenamic acid;    MRC-5 cells;    functionalization;    biocompatibility;   

DOI  :  10.3390/bioengineering5010004

来源: DOAJ

【 摘 要 】

Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug. The present work, aimed at functionalizing MCM-41 (Mobil Composition of Matter No. 41) silica nanoparticles with FFA and determining its biocompatibility with human fibroblasts MRC-5 (Medical Research Council cell strain 5). MCM-41, was synthesized and characterized structurally and chemically, with multiple techniques. The biocompatibility assay was performed by Live/Dead technique, with calcein and propidium–iodide. MRC-5 cells were treated with FFA-grafted MCM-41 for 48 h, and 98% of cells remained viable, without signs of necrosis or morphological changes. The results show the feasibility of MCM-41 functionalization with FFA, and its potential protection of normal cells, in comparison to the role of FFA in cancerous ones.

【 授权许可】

Unknown