Cloning and Expression of Canine Interferon-α Genes in Escherichia coli

Journal of Veterinary Medical Science

Osamu TAIRA²  Masaki TAKAHASHI²  Setsuo ARAI¹  Itsuki WATANUGI²  Nobutoshi MAEHARA²  Hisaaki SATO²  Yuko HAGIWARA² 

[1] The Kitasato Institute;Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University

关键词: canine IFN-α;    cloning;    expression;   

DOI  :  10.1292/jvms.67.1059

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(8)We cloned five new subtypes of cDNA encoding canine interferon-α (CaIFN-α) from a canine epithelial cell line. CaIFN-αs were divided into two groups by amino acid sequences and a molecular phylogenic tree. Two subtypes of them were expressed in Escherichia coli, and IFN proteins were purified. Recombinant CaIFN-αs were highly species-specific and showed antiviral activity against Vesicular stomatitis New Jersey virus and canine adenovirus-1 , but not against canine herpesvirus-1.

【 授权许可】

Unknown