Isolation and Expression of Five-Amino-Acid-Deleted Variant of Feline Hepatocyte Growth Factor (HGF) cDNA

Journal of Veterinary Medical Science

Yoshimi YAMAMOTO²  Shigehiro IWABUCHI¹  Yoshihisa HAGA¹  Koh-ichi SAZE¹  Susumu Y. TAKAHASHI²  Takashi WAKI²  Yoshio ENDO¹  Takahiro YAGUCHI¹  Masayuki OKAZAKI¹  Yasuyuki SUZUTA¹  Toshiyuki OGAWA¹  Janhui WANG¹  Masashi MIYAKE¹ 

[1] Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.;Department of Biochemistry and Radiation Biology, Graduate School of Veterinary Sciences, Yamaguchi University

关键词: cloning;    expression;    feline;    leukocyte;    variant HGF;   

DOI  :  10.1292/jvms.66.9

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(1)Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity.

【 授权许可】

Unknown