Detection of marine birnavirus genome in zooplankton collected from the Uwa Sea, Japan

Diseases of Aquatic Organisms

Shin-Ichi Nakano¹  Satoru Suzuki¹  Shin-Ichi Kitamura¹  Shin-Ichiro Kamata¹ 

关键词: Birnavirus;    Plankton;    Reservoir;    Vector;    Uwa Sea;   

DOI  :  10.3354/dao054069

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Marine birnaviruses (MABVs) infect a wide range of fish and shellfish, yet their mode of transmission is still unclear. To determine whether marine plankton serve as a vector for MABVs, we examined plankton collected from the Uwa Sea, Japan. Thephytoplankton and zooplankton were collected monthly, at depths of 0 and 40 m, from May to November 2001. Detection of the MABV genome was carried out using 2-step PCR and virus isolation. Viral genome was detected in zooplankton collected at 0 m depthin September and at 40 m depth in November. The virus could not be isolated in the PCR-positive samples. These results suggest that zooplankton may act as a vector of MABVs, although the infective and/or accumulated virus titer in zooplankton was low.

【 授权许可】

Unknown