Four years of monitoring for viral haemorrhagic septicaemia virus in marine waters around the United Kingdom

Diseases of Aquatic Organisms

H. K. Strømmen¹  C. Tsin-yee Lui¹  L. Parry¹  E. Chambers¹  J. K. Thurlow¹  S. Feist¹  S. Avery¹  D. M. Stone¹  H. Mandhar¹  K.Way¹  P. F. Dixon¹ 

关键词: Viral haemorrhagic septicaemia virus;    VHSV;    Birnavirus;    Aquareovirus;    Marine fish;    UK;    Survey;   

DOI  :  10.3354/dao054175

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Between 1995 and 1998, marine fish from around the coast of the UK were collected and samples analysed for viral haemorrhagic septicaemia virus (VHSV) using cell culture isolation methods. In 1997 and 1998 the samples were also analysed for VHSVby reverse transcription PCR (RT-PCR). A total of 1867 fish of 11 species were tested, but VHSV was isolated on only 1 occasion, from herring Clupea harengus, in 1996 . However, despite VHSV not being isolated in 1997 and 1998, in both yearssamples of herring from the west and south coasts of England produced positive signals in the RT-PCR, and in 1997 cod from the east coast of England also produced positive signals in the RT-PCR. These results are believed to be true indications of thepresence of VHSV nucleic acid in the fish. In 1997, birnaviruses from Serogroup B1 were isolated from herring (a previously unrecorded host for the virus) and cod Gadus morhua, and a birnavirus from Serogroup A2 was also isolated from cod. In 1998,an aquareovirus was isolated from haddock Melanogrammus aeglefinus, a previously unrecorded host for the virus.

【 授权许可】

Unknown