Susceptibility of juvenile Atlantic cod Gadus morhua to viral haemorrhagic septicaemia virus isolated from wild-caught Atlantic cod

Diseases of Aquatic Organisms

I. R. Bricknell¹  M. Snow¹  C. O. Cunningham¹ 

关键词: Viral haemorrhagic septicaemia virus;    VHSV;    Novirhabdovirus;    Cod;   

DOI  :  10.3354/dao041225

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A European strain of viral haemorrhagic septicaemia virus (VHSV) isolated from wild-caught cod Gadus morhua (H16/7/95) was shown to cause clinical disease and mortality in excess of 80% in juvenile Atlantic cod when administered by theintra-peritoneal (i.p.) route. No virus was recovered from cod cohabiting with experimentally infected fish at a ratio of 1:1, and no VHSV-associated mortality was demonstrated following immersion infection. External signs of disease in cod were thepresence of exophthalmia and ascites. Virus was identified as VHSV by enzyme-linked immunosorbent assay (ELISA) and was recovered from both brain and organ pools (kidney, liver and spleen) of 100% of i.p. infected cod mortalities. Virus was also detectedusing an indirect immunofluorescence test on tissue imprints of kidney, liver, spleen and brain taken from moribund fish. The fact that cod were not susceptible to VHSV following waterborne exposure raises important questions surrounding the propagation,maintenance and impact of a naturally occurring reservoir of virus in the marine environment.

【 授权许可】

Unknown