Visible, Safe and Certain Endotracheal Intubation Using Endoscope System andInhalation Anesthesia for Rats

Journal of Veterinary Medical Science

Naoki ITANO⁶  Yumi SHIOTANI¹  Kyoko SHIOYA⁵  Noriyuki KASAI⁴  Kenjiro KONNO¹  Teppei OGAWA³  Mika HATAKEYAMA² 

[1] Department of Animal Medical Sciences, Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo, Kita-ku, Kyoto 603-8555, Japan;AVS Co., Ltd., Shinjuku San-ei Bldg. 5th Fl., 1�?22�?2 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan;NATSUME SEISAKUSHO CO., LTD., 7�?18 Saitoasagi-7, Ibaraki-shi, Osaka 567-0085, Japan;Center for Laboratory of Animal Research, Tohoku University, 2�?1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan;Laboratory of Animal Experiment and Medicine Management, National Cerebral and Cardiovascular Center, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan;Institute of Advanced Technology, Kyoto Sangyo University, Kamigamo, Kita-ku, Kyoto 603-8555, Japan

关键词: 3Rs;    anesthesia;    endoscope;    intubation;    rat;   

DOI  :  10.1292/jvms.14-0146

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(1)Anesthesia strongly influenceslaboratory animals, and it can also greatly affect the experimental data. Rats rank onlysecond to mice in the number used in research fields, such as organ transplantation,regenerative medicine and imaging. Therefore, appropriate and effective anesthesia,including the protocol of the endotracheal intubation and inhalation anesthesia, iscrucial. Hence, we evaluated these methods in this study. Twelve Wistar rats wereintraperitoneally injected with M/M/B: 0.3/4/5, comprising of medetomidine, midazolam andbutorphanol at a dose of 0.3 mg/kg + 4.0 mg/kg + 5.0 mg/kg body weight/rat, respectively.An endotracheal tube was then intubated into the trachea. After intubation, the rats wereconnected to the inhalation anesthesia circuit using isoflurane, and vital signs weremeasured until 30 min after connection. All intubations were successfully finished within1 min, and the values of the vital signs were normal and stable. In addition,histopathological observation of the trachea and lungs showed no trauma. These resultssuggest that this visible endotracheal intubation method is simple, reliable, safe andfavorable with regard to the rats�? welfare.

【 授权许可】

Unknown