Relationship between Bacterial Translocation and Isolation of Enteric Bacteria from Extraintestinal Organs in Slaughtered Pigs

Journal of Veterinary Medical Science

Mio KANAZAWA²  Akihiro OGAWA⁴  Masahito SUGISHIMA³  Takemi OHBA¹  Satoshi MURAKAMI² 

[1] Toyama Prefectural Meat Inspection Center;Department of Animal Science, Tokyo University of Agriculture;Kanagawa Prefectural Meet Inspection Center;Chuo Animal Health and Hygiene Service Center

关键词: ascariasis;    bacterial translocation;    enteric bacteria;    gastrointestinal tract;    swine;   

DOI  :  10.1292/jvms.11-0154

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(1)Enteric bacteria, especially Escherichia coli, have been isolated from porcine livers affected with ascariasis. We hypothesized that reason for this bacterial isolation was due to bacterial translocation (BT). In order to clarify the association between ascariasis and isolation of Enterobacteriaceae in the livers,12 pigs with ascariasis (ascariasis group), 12 pigs without ascariasis (non-ascariasis A group) were used. Jejunum, ileum, cecum, colon, livers, mesenteric lymph nodes (MLN), and portal blood were obtained from these pigs. Furthermore, in order to confirm the presence of BT in pigs, the samples mentioned above as well as spleens were obtained from 11 pigs without ascariasis (non-ascariasis B group) and 6 specific pathogen free (SPF) pigs without ascariasis (non-ascariasis SPF group). In the first experiment, the Gram-negative bacteria were isolated from livers (66.7%), MLN (91.7%), and portal blood (55.6%) regardless the presence of ascariasis. In the second experiment, isolation rates of Gram-negative and -positive bacteria were 52.9 and 66.7% for livers, 52.9 and 80% for MLN, 11.8 and 26.7% for spleens, and 40 and 20% for portal blood of the pigs examined, respectively. E. coli and Staphylococcus were the predominant isolates from these pigs. A large number of antigens immunodetected by E. coli polyclonal antibody were found in the damaged cecal mucosa. These findings present evidence that BT is generally observed in slaughtered pigs regardless the presence of ascariasis. This has challenged our notion that extraintestinal organs of pigs are usually maintained as sterile.

【 授权许可】

Unknown