Temporal and Topographic Profiles of Tissue Hypoxia Following Transient Focal Cerebral Ischemia in Rats

Journal of Veterinary Medical Science

Takahisa NOTO¹  Ichiro ARAMORI¹  Yasuhisa FURUICHI¹  Masayuki ISHIYE¹  Noboru MANABE²  Takehiko YANAGIHARA³  Seitaro MUTOH¹  Nobuya MATSUOKA¹ 

[1]Medicinal Biology Research Laboratories, Astellas Pharmaceutical Inc.

[2]Research Unit for Animal Life Sciences, Animal Resource Science Center, The University of Tokyo

[3]Osaka Neurological Research Institute

关键词: granulocyte;    hypoxia;    immunohistochemistry;    platelet;    rat;   

DOI  :  10.1292/jvms.68.803

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(9)Intravascular accumulation of blood cells after brain ischemia-reperfusion can cause obstruction of cerebral blood flow and tissue hypoxia/ischemia as a consequence. In the present study, we examined temporal and topographic changes of tissue hypoxia/ischemia after occlusion of the middle cerebral artery (MCA) for 60 min in rats with immunohistochemical staining for hypoxia (2-nitroimidazole hypoxia marker: hypoxyprobe-1 adducts). Our results showed that tissue hypoxia expressed as positive staining for hypoxyprobe-1 adducts preceded neuronal degeneration. Platelets and granulocytes were detected close to the hypoxyprobe-1 adducts positive area. These results suggested that the hypoxic environment could persist even after reperfusion of MCA, because of vascular obstruction with accumulation of platelets and granulocytes.

【 授权许可】

Unknown