Development of unidentified dna-specific hif 1α gene of lizard (hemidactylus platyurus) which plays a role in tissue regeneration process

3rd International Conference on Biological Sciences and Biotechnology

Novianti, T.^1 ; Sadikin, M.^2 ; Widia, S.^2 ; Juniantito, V.^3 ; Arida, E.A.^4

Biotechnology Department, Esa Unggul Universiy, Jl. Raya Arjuna Utara no. 9, Jakarta Barat, Indonesia^1

Biochemestry Department, Faculty of Health, University of Indonesia, Jl.raya salemba no 6, Jakarta Pusat, Indonesia^2

Patology and Anatomy Department, Faculty of Veteriner, Institute of Agriculture Bogor, Jl.Dramaga Bogor, Indonesia^3

Puslit Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Jl.Raya Cibinong, Bogor, Indonesia^4

关键词: Basic local alignment sequence tools;    Biological process;    Chi-square statistic test;    hypoxia;    Multiple alignment;    On-line softwares;    PCR techniques;    Regeneration process;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/130/1/012031/pdf
DOI  :  10.1088/1755-1315/130/1/012031

来源: IOP

【 摘 要 】

Development of unidentified specific gene is essential to analyze the availability these genes in biological process. Identification unidentified specific DNA of HIF 1α genes is important to analyze their contribution in tissue regeneration process in lizard tail (Hemidactylus platyurus). Bioinformatics and PCR techniques are relatively an easier method to identify an unidentified gene. The most widely used method is BLAST (Basic Local Alignment Sequence Tools) method for alignment the sequences from the other organism. BLAST technique is online software from website https://blast.ncbi.nlm.nih.gov/Blast.cgi that capable to generate the similar sequences from closest kinship to distant kindship. Gecko japonicus is a species that it has closest kinship with H. platyurus. Comparing HIF 1 α gene sequence of G. japonicus with the other species used multiple alignment methods from Mega7 software. Conserved base areas were identified using Clustal IX method. Primary DNA of HIF 1 α gene was design by Primer3 software. HIF 1α gene of lizard (H. platyurus) was successfully amplified using a real-time PCR machine by primary DNA that we had designed from Gecko japonicus. Identification unidentified gene of HIF 1a lizard has been done successfully with multiple alignment method. The study was conducted by analyzing during the growth of tail on day 1, 3, 5, 7, 10, 13 and 17 of lizard tail after autotomy. Process amplification of HIF 1α gene was described by CT value in real time PCR machine. HIF 1α expression of gene is quantified by Livak formula. Chi-square statistic test is 0.000 which means that there is a different expression of HIF 1 α gene in every growth day treatment.