Generation of Monoclonal Antibody against Canine Neural-Cell Adhesion Molecule

Journal of Veterinary Medical Science

Makoto BONKOBARA¹  Naoaki MATSUKI⁴  Ayumi KOIKE⁵  Hiroko YAGIHARA¹  Tomohiro YAMAGUCHI⁵  Yosuke UEMATSU⁵  Daisuke HASEGAWA²  Kenichiro ONO⁴  Tsukimi WASHIZU¹  Toshiro ARAI³ 

[1] Department of Veterinary Clinical Pathology, Nippon Veterinary and Life Science University;Department of Veterinary Radiology, Nippon Veterinary and Life Science University;Department of Veterinary Biochemistry, Nippon Veterinary and Life Science University;Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Canine-Lab. Inc.

关键词: canine;    monoclonal antibody;    neural-cell adhesion molecule;   

DOI  :  10.1292/jvms.70.845

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)A monoclonal antibody, K9BYU, was generated using Escherichia coli recombinant extracellular domain of canine neural-cell adhesion molecule (N-CAM) as an antigen. Immunoreactivity of K9BYU to insect cell recombinant canine N-CAM was demonstrated by Western blotting using Sf9 insect cells transfected with the canine N-CAM gene. In Western blotting against canine brain tissue, K9BYU detected three isoforms of N-CAM that correspond to three major isoforms of human and mouse N-CAM (N-CAM-120, -140, and -180). From these results, K9BYU was considered to be a useful tool for research of canine N-CAM.

【 授权许可】

Unknown