Caveolae/Caveolin-1 Are Important Modulators of Store-Operated Calcium Entry in Hs578/T Breast Cancer Cells

Journal of Pharmacological Sciences

Ping Wu²  Hua Zhu¹  Noah Weisleder³  Chuanxi Cai³  Jian-wen Chen² 

[1] The Cancer Institute of New Jersey, University of Medicine and Dentistry of New Jersey, USA;Center of Systems Biology, Institute of Biophysics, Chinese Academy of Sciences, China;Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, USA

关键词: caveolae;    caveolin-1;    store-operated calcium channel;    tumor progression;   

DOI  :  10.1254/jphs.FP0071192

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(41)Cited-By(4)Caveolin-1 is a principal component of caveolae, invaginations of the plasma membrane that are enriched in cholesterol and sphingolipids. The expression of caveolin-1 has been shown to be tightly correlated to the progression of breast cancer tumors. However, the consequences of altered caveolin-1 expression during tumor progression still remain unclear. Modification of caveolin-1 expression modulates store-operated Ca2+ entry (SOCE) in various cell types. SOCE is a ubiquitous Ca2+ entry pathway that previous studies have linked to apoptosis and tumor progression in prostate cancer cells. In this study, we tested the effect of altering caveolin-1 expression on SOCE in Hs578/T breast cancer cells. Through overexpression of caveolin-1 and small hairpin RNA (shRNA) knockdown, we generated four stable cell lines that have 3 different caveolin-1 protein levels. Cav-1 overexpression could increase SOCE activity, while knockdown of caveolin-1 significantly reduced SOCE activity. These functional consequences were correlated with changes in caveolae number in Hs578/T cells. Our results suggest alteration of SOCE by caveolin-1 expression changes could be one of the mechanisms contributing to the progression of breast cancer.

【 授权许可】

Unknown