Identification of Proteins Regulated by Ferulic Acid in a Middle Cerebral Artery Occlusion Animal Model-A Proteomics Approach

Journal of Veterinary Medical Science

Eun-Hae CHO¹  Jae-Hyeon CHO¹  Myeong-Ok KIM²  Chung-Kil WON¹  Jin-Hee SUNG¹  Phil-Ok KOH¹ 

[1] Department of Anatomy, College of Veterinary Medicine and Research Institute of Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju 660–701, South Korea;Division of Life Science and Applied Life Science (Brain Korea 21), Gyeongsang National University, 900 Gajwa-dong, Jinju 660–701, South Korea

关键词: ferulic acid;    ischemia;    proteomics;   

DOI  :  10.1292/jvms.12-0063

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Ferulic acid plays a neuroprotective role in cerebral ischemia. The aim of this study was to identify the proteins that are differentially expressed following ferulic acid treatment during ischemic brain injury using a proteomics technique. Middle cerebral artery occlusion (MCAO) was performed to induce a focal cerebral ischemic injury in adult male rats, and ferulic acid (100 mg/kg) or vehicle was administered immediately after MCAO. Brain tissues were collected 24 hr after MCAO. The proteins in the cerebral cortex were separated using two-dimensional gel electrophoresis and were identified by mass spectrometry. We detected differentially expressed proteins between vehicle- and ferulic acid-treated animals. Adenosylhomocysteinase, isocitrate dehydrogenase [NAD+], mitogen-activated protein kinase kinase 1 and glyceraldehyde-3-phosphate dehydrogenase were decreased in the vehicle-treated group, and ferulic acid prevented the injury-induced decreases in these proteins. However, pyridoxal phosphate phosphatase and heat shock protein 60 were increased in the vehicle-treated group, while ferulic acid prevented the injury-induced increase in these proteins. It is accepted that these enzymes are involved in cellular metabolism and differentiation. Thus, these findings suggest evidence that ferulic acid plays a neuroprotective role against focal cerebral ischemia through the up- and down-modulation of specific enzymes.

【 授权许可】

Unknown