Proteomic Identification of Proteins Differentially Expressed in Response toResveratrol Treatment in Middle Cerebral Artery Occlusion Stroke Model

Journal of Veterinary Medical Science

Myeong-Ok KIM¹  Phil-Ok KOH²  Fawad-Ali SHAH²  Sang-Ah GIM² 

[1] Division of Life Science and Applied Life Science, Gyeongsang National University, 900 Gajwa-dong, Jinju 660-701, South Korea;Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea

关键词: ischemia;    proteomics;    rat;    resveratrol;   

DOI  :  10.1292/jvms.14-0169

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(1)Resveratrol has aneuroprotective effect against cerebral ischemia. The objective of this study was toidentify proteins that are differentially expressed in the cerebral cortex of vehicle- andresveratrol-treated animals during ischemic injury. Focal cerebral ischemia was induced asmiddle cerebral artery occlusion (MCAO) in male rats. Rats were treated with vehicle orresveratrol before MCAO, and cerebral cortex was collected 24 hr after MCAO. Cerebralcortex proteins were identified by two-dimensional gel electrophoresis and massspectrometry. Several proteins were identified as differentially expressed betweenvehicle- and resveratrol-treated animals. Among these proteins, expression ofperoxiredoxin-5, isocitrate dehydrogenase [NAD+], apolipoprotein A-I andubiquitin carboxy terminal hydrolase L1 was decreased in the vehicle-treated group,whereas resveratrol attenuated the injury-induced decrease in expression of theseproteins. However, expression of collapsing response mediator protein 2 was increased inthe vehicle-treated group, whereas resveratrol prevented the injury-induced increase inthe expression of this protein. These findings suggest that resveratrol modulates theexpression of various proteins that associated with oxidative stress and energy metabolismin focal cerebral ischemia.

【 授权许可】

Unknown