Molecular Cloning of Canine nm23 cDNAs and Their Expression in Normal and Tumor Tissues

Journal of Veterinary Medical Science

Noriaki MIYOSHI⁴  Akihiro OHISHI²  Kazuhiro MISUMI¹  Kenjiro FUKUSHIMA³  Masashi TAKAHASHI³  Yasuyuki ENDO³  Masao AKUZAWA³  Ryoma UNE³  Makoto FUJIKI¹ 

[1] Laboratory of Veterinary Surgery, Faculty of Agriculture, Kagoshima University;Laboratory of Veterinary Animal Hospital, Faculty of Agriculture, Kagoshima University;Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Kagoshima University;Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University

关键词: canine;    NM23;    tumor;   

DOI  :  10.1292/jvms.67.837

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(2)Two canine nm-23 cDNAs, designated as nm23-C1 and -C2, were isolated and characterized. Both have a putative open reading frame consisting of 459-bp encoding 152 amino acids and are highly similar to human, mouse and rat homologues. To understand the potential role of nm23-C1 and -C2 in the development of mammary gland tumors (MGT), we analyzed the mRNA expression in 14 MGT samples by RT-PCR. The samples were divided into categories according to their histopathology (benign/malignant) and metastasis. No significant difference in the mRNA expression levels of either nm23-C1 or -C2 were observed between the benign and malignant groups or the metastatic and non-metastatic groups. These results suggest that nm23-C1 and -C2 are not related to the establishment of malignancy and metastatic lesions in canine MGT cases.

【 授权许可】

Unknown