Bulgarian Propolis Induces Analgesic and Anti-inflammatory Effects in Mice and Inhibits In Vitro Contraction of Airway Smooth Muscle

Journal of Pharmacological Sciences

João Batista Calixto¹  Amarilis Scremin²  Daniela Taggliari Longhi²  Andreia Pires Dantas⁴  Niraldo Paulino²  Solange Lisboa de Castro⁴  Vassya Bankova³ 

[1] Departamento de Farmacologia, Universidade Federal de Santa Catarina;Grupo de Pesquisa e Desenvolvimento de Biofármacos (BIOFAR), Universidade do Sul de Santa Catarina;Institute of Organic Chemistry with Centre of Phytochemistry;Departamento de Ultra-estrutura e Biologia Celular, Fundação Oswaldo Cruz

关键词: propolis;    anti-nociception agent;    anti-inflammatory agent;    guinea pig trachea;    antispasmodic effect;   

DOI  :  10.1254/jphs.93.307

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(39)Cited-By(34)Propolis is a bee product, which has long been used in folk medicine for the management of different diseases. In this study we evaluated the analgesic and anti-inflammatory effects of a standard ethanolic extract of Bulgarian propolis (Et-Blg) in mice and its in vitro effect on airway smooth muscle. Et-Blg inhibited acetic acid-induced abdominal contortions with an ID50 = 7.4 ± 0.7 mg · kg−1. In the formalin test, the extract caused a significant reduction in pain in mice treated with 100 mg · kg−1 Et-Blg during the neurogenic phase and for the inflammatory phase with all doses of the extract, with an ID50 = 2.5 ± 0.4 mg · kg−1. Et-Blg inhibited also the capsaicin-induced ear edema in mice; however, this extract was ineffective when assessed in the tail-flick and hot-plate thermal assays. The analgesic effect of Et-Blg was associated with the inhibition of inflammatory responses and not to a simple irritation of nervous terminals. In vitro, this extract inhibited the contraction of trachea smooth muscle induced by histamine (IC50 = 50 ± 5 μg · mL−1), capsaicin (IC50 = 26.8 ± 3 μg · mL−1), 80 mM KCl (IC50 = 27.8 ± 3 μg · mL−1), and carbachol (IC50 = 54 ± 2 μg · mL−1).

【 授权许可】

Unknown