Flow Cytometric Analysis for Enterotoxin Exposed on Clostridium Perfringens Spores

Journal of Veterinary Medical Science

Dong HU¹  Ramani H. PIYANKARAGE¹  Hirofumi KUSUNOKI¹  Shunji SUGII¹  Takashi UEMURA¹ 

[1] Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

关键词: Clostridium perfringens;    enterotoxin;    FCM;   

DOI  :  10.1292/jvms.60.1357

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)Cited-By(3)Flow cytometric method (FCM) with fluorescent-labeled anti-CPE antibody was applied to develop a rapid, specific, and convenient method to detect enterotoxin (CPE) exposed on the surface of spores of Clostridium perfringens. The results obtained indicate that FCM can specifically detect CPE exposed on C. perfringens spores for a short time. Thus, FCM is found to be a rapid, specific, and convenient assay method for detection of CPE exposed on C. perfringens spores, suggesting that it will be hopefully useful to diagnose food poisoning caused by C. perfringens.

【 授权许可】

Unknown