Evaluation of the Chondroprotective Effects of Glucosamine and Fish Collagen Peptide on a Rabbit ACLT Model Using Serum Biomarkers

Journal of Veterinary Medical Science

Akihiro OHNISHI²  Tomohiro IMAGAWA²  Saburo MINAMI²  Yoshiharu OKAMOTO²  Tomohiro OSAKI²  Yoshiharu MATAHIRA¹  Takeshi TSUKA² 

[1] R & D Division, Yaizu Suisankagaku Industry Co., Ltd., 5–8–13 Kogawa-Shinmachi, Yaizu 425–8570, Japan;Department of Veterinary Clinical Medicine, Tottori University, 4–101 Koyama-Minami, Tottori 680–8553, Japan

关键词: biomarkers;    fish collagen peptide;    glucosamine;    osteoarthritis;   

DOI  :  10.1292/jvms.12-0240

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(3)The aim of this study was to investigate the correlations of severity of osteoarthritis (OA) and serum biomarkers including keratan sulfate (KS), hyaluronic acid (HA) and chondroitin sulfate (CS) 846 epitope. We also investigated the effect of glucosamine and fish collagen peptide (FCP) on OA. OA was induced in 12 rabbits (12 weeks of age) by anterior cruciate ligament transection (ACLT). After the surgery, the rabbits were orally administered FCP (F group), glucosamine (G group) or FCP and glucosamine (FG group) for 4 weeks. The control group was provided water ad libitum (C group). Blood was collected before surgery (pre-ACLT) and before euthanasia (post-ACLT) for serum marker measurement. Biomarker levels were measured by using commercial kits. We evaluated OA severity both macroscopically and histologically. Macroscopic evaluation showed mildly eroded condylar surfaces in the C group. Histological findings were significantly different from the FG and other groups. There were no significant differences between each group at post-ACLT in terms of serum KS, HA and CS 846. Histological assessment and serum biomarker measurements performed at post-ACLT showed a significant correlation between HA concentration and OA severity. Variations in the CS 846 concentration at pre-ACLT and post-ACLT were significantly correlated with OA severity. Administration of glucosamine and FCP had chondroprotective effects in the ACLT model. Serum biomarker concentrations were significantly correlated with cartilage injury. Serum biomarker measurement would be useful for monitoring articular cartilage damage in the clinical setting.

【 授权许可】

Unknown