Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis

Diseases of Aquatic Organisms

Janet K. Epp¹  John Wood¹  Eric P. Bergersen¹  George J. Schisler¹  Peter G. Walker¹ 

关键词: Whirling disease;    Myxobolus cerebralis;    Pepsin-trypsin digest (PTD);    Polymerase chain reaction (PCR);    Testing methods;   

DOI  :  10.3354/dao045109

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids.Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of theparasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p < 0.001) with a subjective evaluation of amplicon staining intensity.

【 授权许可】

Unknown