Validation of a single round polymerasechain reaction assay for identification ofMyxobolus cerebralis myxospores

Diseases of Aquatic Organisms

Karin A. Myklebust¹  Thomas J. Baldwin¹ 

关键词: Myxobolus cerebralis;    Whirling disease;   

DOI  :  10.3354/dao049185

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Validation of a single round PCR-based assay to confirm as Myxobolus cerebralis myxospores obtained from pepsin-trypsin digest preparations is described.The assay is a modification of a PCR assay published previously, based on theamplification of a segment of the gene encoding the 18S ribosomal subunit of M. cerebralis.The sensitivity, specificity and upper and lower detection limits were determined using known M. cerebralis and non-M. cerebralis myxosporesand M. cerebralis-free fish.The sensitivity of PCR confirmation was 100% (95% confidence interval of 83.2-100%).The specificity was 100% (95% confidence interval of 87.2-100%).The upper detection limit was approximately 100000 myxospores perreaction; the lower detection limit was approximately 50 myxospores per reaction.Given the high sensitivity and specificity of the assay, substitution of this assay for histologic confirmation of M. cerebralis infection is encouraged.

【 授权许可】

Unknown