Bovine Viral Diarrhea Virus Replication in Bovine Follicular Epithelial Cells Derived from Persistently Infected Heifers

Journal of Veterinary Medical Science

Takamitsu TSUBOI¹  Tadao IMADA² 

[1] Laboratory of Microbiological Diagnosis, Department of Systematic Diagnosis, National Institute of Animal Health;Laboratory of viral Pathogenesis, Department of Virology, National Institute of Animal Health

关键词: bovine viral diarrhea virus;    follicular epithelial cell;    follicular fluid;    in vitro fertilization;    persistent infection;   

DOI  :  10.1292/jvms.60.569

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(15)Bovine follicle fluid and oocytes surrounded by follicular epithelial (FE) cells were collected from ovaries of two heifers persistently infected with bovine viral diarrhea virus (BVDV). BVDV was present in the follicle fluid at a higher titer than in serum. The oocytes were matured in vitro under culture conditions of 39°C in humidified air containing 5% CO2. In vitro fertilization was performed after 24 hr in culture (the day of insemination was defined as day l), and culture was continued through day 10. BVDV was present in the culture medium at titers of 102.25 to 103.25 TC ID50/0.l ml. The virus was also detected in FE cells collected on day 10. Viral antigen was demonstrated in the cytoplasm of FE cells by the indirect immunofluorescence technique. However, no BVDV was detected in the embryos on day 10. These findings suggested that the oocytes or embryos were unlikely to be infected with BVDV, but that the FE cells were infected with BVDV and supported virus replication in cattle persistently infected with BVDV.

【 授权许可】

Unknown