Bovine Herpes Virus Expressing Envelope Protein (E2) of Bovine Viral Diarrhea Virus As a Vaccine Candidate

Journal of Veterinary Medical Science

Seung-Won Kang¹  Chang-Hee Kweon¹  Eun-Jin Choi¹  Yung-Bai Kang¹ 

[1] KANGNational Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Republic of Korea

关键词: BVDV;    expression of E2 protein;    TK- BHV-1 recombinant;    viral vector;   

DOI  :  10.1292/jvms.61.395

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(9)The gene encoding the envelope protein (E2) of bovine viral diarrhea virus (BVDV) was expressed under the thymidine kinase (TK) promoter of Korean bovine herpesvirus 1 (BHV-1) isolate. Thymidine kinase negative (TK-) BHV-1 recombinants expressing E2 of BVDV were constructed and the expression of E2 was identified by immunofluorescence and Western blotting. Compared to wild type BHV-1, the recombinant BHV-1 had a delayed cytopathogenic effect in cells. The immunogenicity of the recombinant BHV-1 was examined in guinea pigs and cattle. Although an increase in body temperature was detected for a few days, the inoculated cattle returned to normal temperature with the development of neutralizing antibodies to BVDV.

【 授权许可】

Unknown