Cloning and Characterization of a 2-Cys Peroxiredoxin fromBabesia gibsoni

Journal of Veterinary Medical Science

Madoka ICHIKAWA-SEKI¹  Miho USUI³  Kei HAYASHI³  Masahito ASADA²  Mohamad A. TERKAWI³  Shin-ichiro KAWAZU³  Xuenan XUAN³  Tatsunori MASATANI³ 

[1] Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3�?18�?8 Ueda, Morioka, Iwate 020�?8550, Japan;Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1�?12�?4 Sakamoto, Nagasaki 852�?8523, Japan;National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080�?8555, Japan

关键词: antioxidant activity;    Babesia gibsoni;    canine;    peroxiredoxin;   

DOI  :  10.1292/jvms.13-0274

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(32)Cited-By(4)Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a2-Cys Prx, BgTPx-1, from the canine Babesia parasiteB. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx ofB. bovis was 81% at the amino acid level. Enzyme activity assay byusing recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity.Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect aprotein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be aninefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B.gibsoni merozoites. These results suggest that the BgTPx-1 may play a role tocontrol redox balance in the cytoplasm of B. gibsoni.

【 授权许可】

Unknown