Transformation of chlorophyll a during viral infection of Emiliania huxleyi

Aquatic Microbial Ecology

Susan A. Kimmance¹  Nicole J. Bale¹  Carole A. Llewellyn¹  Ruth L. Airs¹ 

关键词: Viral infection;    Emiliania huxleyi;    Chlorophyll a;    Transformation products;    Oxidative transformation;   

DOI  :  10.3354/ame01640

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Although viruses can significantly reduce primary production-mediated carbon cycling, the effect of viral infection on the principal photosynthetic pigment that enables autotrophic production, chlorophyll a (chl a), remains unresolved. We compared the production of chl a transformation compounds in Emiliania huxleyi cultures undergoing viral infection with that in control cultures left to decline in the stationary phase of growth. A high performance liquid chromatography mass spectrometry method developed for the detection of Type I chl a transformation products was used. A rapid decline in cellular concentrations of chl a, C-13² diastereomer of chl a (chl a�?) and phaeophytin a (phytin a) was observed in both infected and control cultures. The most notable finding was the significant increase in the cellular concentrations of Type I chl oxidation products (allomers) in the infected cultures, and we hypothesise that this may be due to increased oxidative stress and reactive oxygen species.

【 授权许可】

Unknown